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Developmental Biology Protocols: Volume III by Rocky S. Tuan (English) Hardcover

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Objectstaat: Nieuw: Een nieuw, ongelezen en ongebruikt boek in perfecte staat waarin geen bladzijden ontbreken of ...

ISBN-13: 9780896038547

Book Title: Developmental Biology Protocols

ISBN: 9780896038547

Publication Name: Developmental Biology Protocols

Item Length: 10in

Publisher: Humana Press

Publication Year: 2000

Series: Methods in Molecular Biology Ser.

Type: Textbook

Format: Hardcover

Language: English

Item Height: 0.5in

Author: Cecilia W. Lo

Item Width: 7in

Item Weight: 48.1 Oz

Number of Pages: Xxvi, 522 Pages

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Product Information

Developmental biology is one of the most exciting and fast-growing fields today. In part, this is so because the subject matter deals with the innately fascinating biological events--changes in form, structure, and function of the organism. The other reason for much of the excitement in developmental biology is that the field has truly become the unifying melting pot of biology, and provides a framework that integrates anatomy, physiology, genetics, biochemistry, and cellular and molecular biology, as well as evolutionary biology. No longer is the study of embryonic development merely "embryology." In fact, development biology has produced important paradigms for both basic and clinical biomedical sciences. Though modern developmental biology has its roots in "experimental embry- ogy" and the even more classical "chemical embryology," the recent explosive and remarkable advances in developmental biology are critically linked to the advent of the "cellular and molecular biology revolution." The impressive arsenal of expe- mental and analytical tools derived from cell and molecular biology, which promise to continue to expand, together with the exponentially developing sophistication in fu- tional imaging and information technologies, guarantee that the study of the devel- ing embryo will contribute one of the most captivating areas of biological research in the next millennium.

Product Identifiers

Publisher

Humana Press

ISBN-10

0896038548

ISBN-13

9780896038547

eBay Product ID (ePID)

227038

Product Key Features

Author

Cecilia W. Lo

Publication Name

Developmental Biology Protocols

Format

Hardcover

Language

English

Publication Year

2000

Series

Methods in Molecular Biology Ser.

Type

Textbook

Number of Pages

Xxvi, 522 Pages

Dimensions

Item Length

10in

Item Height

0.5in

Item Width

7in

Item Weight

48.1 Oz

Additional Product Features

Series Volume Number

137

Number of Volumes

1 Vol.

Lc Classification Number

Qh491-492

Volume Number

Vol. 3

Reviews

"We live in a time of rising expectations. Being a developmental biologist at the turn of the millenium demands expertise in embryological manipulation, viral gene expression, in situ hybridization, videomicroscopy, in vitro assays, transgenic embryo production, cell lineage analysis, PCR techniques, and computer-enhanced imaging technology. Rocky Tuan and Cecilia Lo have done the field (and each scientist within it) a wonderful service by collecting and editing the protocols of the masters of each echnique. More than 100 papers spanning these areas (and more) patiently lead one through each method, giving details on what brand of microscope slides to use, what centrifuge tubes work best, and where to purchase each piece of equipment. Copious notes provide details based on the laboratories' experiences of what works and what doesn't. Developmental Biology Protocols is a library in itself and will be essential for every laboratory of developmental biology. This is the collection to get before your next grant application is due."-Scott Gilbert, Department of Biology, Swarthmore College, Swarthmore, PA "The range of techniques, from viral vectors to imaging, and of organisms, from sea urchins to mammals, is comprehensive yet focused. This work should be an invaluable aid to those interested in all aspects of comparative development. This work is logically organized and presents a great opportunity for not only picking up a technique but also placing it in a logical context. Well done!" -Barbara B. Knowles, Director of Research, Jackson Labs, Bar Harbor, ME "Among similar laboratory manuals Developmental Biology Protocols edited by Tuan and Lo impress by their breadth of coverage, timeliness, and scrupulous attention to detail. . .these protocols will be an invaluable aid to every geneticist and developmental biologist."-Davor Solter, Director of Developmental Biology, Max-Planck Institute, Freiburg, Germany

Table of Content

Developmental Biology Protocols.- Manipulation Of Developmental Gene Expression and Function.- Ectopic Expression in Drosophila.- Clonal Analysis in the Examination of Gene Function in Drosophila.- Application of Antisense Oligodeoxynucleotides in Developing Chick Embryos.- Application of Functional Blocking Antibodies.- Analysis of Gene Expression.- Gene Expression Analyzed by Ribonuclease Protection Assay.- Relative Reverse Transcription-Polymerase Chain Reaction.- Gene Expression Analysis Using Quantitative Reverse Transcription-Polymerase Chain Reaction and a Multispecific Internal Control.- In Situ PCR Detection of HIV Expression in the Human Placenta.- Gene Expression Analysis by In Situ Hybridization.- Radio-Isotopic In Situ Hybridization on Tissue Sections.- mRNA and Protein Co-Localization on Tissue Sections by Sequential, Colorimetric In Situ Hybridization and Immunohistochemistry.- Whole Mount In Situ Hybridization to Study Gene Expression During Mouse Development.- Multicolor Whole-Mount In Situ Hybridization.- Methods for Double Detection of Gene Expression.- Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins.- Monoclonal Antibodies in the Analysis of Embryonic Development.- Models of Morphogenesis and Development.- Mesoderm Induction in Xenopus.- Amphibian Organizer Activity.- Improved Techniques for Avian Embryo Culture, Somite Cell Culture, and Microsurgery.- Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration.- The Chimeric Human/Mouse Model of Angiogenesis.- Analysis of Embryonic Vascular Morphogenesis.- Epithelial-Mesenchymal Interactions.- Methods for Manipulating the Chick Limb Bud to Study Gene Expression, Tissue Interactions, and Patterning.- Palate Development.- In Vitro Models and Analysis of Differentiation and Development.- In Vitro Fertilization.- Trophoblast Differentiation.- Bone Marrow-Derived Mesenchymal Progenitor Cells.- Identification, Characterization, and Differentiation of Human Prostate Cells.- Preparation of Chick Striated Muscle Cultures.- Study of Skeletal Myogenesis in Cultures of Unsegmented Paraxial Mesoderm.- Embryonic Limb Mesenchyme Micromass Culture as an In Vitro Model for Chondrogenesis and Cartilage Maturation.- Electroporation-Mediated DNA Transfection of Embryonic Chick Limb Mesenchymal Cells.- Murine C3H10T1/2 Multipotential Cells as an In Vitro Model of Mesenchymal Chondrogenesis.- Skeletogenesis.- Studying Early Hematopoiesis Using Avian Blastoderm Cultures.- Isolation and Culture of Mouse Germ Cells.- Cadherin-Mediated Cell-Cell Interactions.- Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Proteins.- Microinjection of Fluorescently Labeled ?-Actinin into Living Cells.- Developmental Models Of Diseases.- Pax3 and Vertebrate Development.- Genetic-Engineered Models of Skeletal Diseases I.- Genetic-Engineered Models of Skeletal Diseases II.- Transgenic Mouse Models of Craniofacial Disorders.

2000

Topic

Life Sciences / Developmental Biology

Dewey Decimal

616.99/465

Intended Audience

Scholarly & Professional

Dewey Edition

Illustrated

Yes

Genre

Science

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