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Methods in Molecular Biology Ser.: MicroRNAs and the Immune System : Methods and

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Specificaties

Objectstaat
Goed: Een boek dat is gelezen, maar zich in goede staat bevindt. De kaft is zeer minimaal beschadigd ...
Subject Area
Science, Medical
ISBN
9781607618102
Publication Name
Micrornas and the Immune System : Methods and Protocols
Publisher
Humana Press
Item Length
10 in
Subject
Immunology, Life Sciences / Biochemistry
Publication Year
2010
Series
Methods in Molecular Biology Ser.
Type
Textbook
Format
Hardcover
Language
English
Author
Silvia Monticelli
Item Weight
29.8 Oz
Item Width
7 in
Number of Pages
Xiii, 330 Pages

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Product Information

This book explores the latest techniques for studying miRNA expression, including the most up-to-date data on splinted ligation and qRT-PCR assays, as well as high-throughput profiling through cloning, deep sequencing, and microarrays.

Product Identifiers

Publisher
Humana Press
ISBN-10
1607618109
ISBN-13
9781607618102
eBay Product ID (ePID)
111014548

Product Key Features

Number of Pages
Xiii, 330 Pages
Language
English
Publication Name
Micrornas and the Immune System : Methods and Protocols
Publication Year
2010
Subject
Immunology, Life Sciences / Biochemistry
Type
Textbook
Subject Area
Science, Medical
Author
Silvia Monticelli
Series
Methods in Molecular Biology Ser.
Format
Hardcover

Dimensions

Item Weight
29.8 Oz
Item Length
10 in
Item Width
7 in

Additional Product Features

Intended Audience
Scholarly & Professional
Reviews
From the reviews:This book is useful to scientists seeking to study the role of miRNAs in the immune system. … helpful to immunologists, biochemists, and molecular biologists interested in miRNA effects. The detailed protocols are invaluable to students and post-docs in particular. … The figures of the types of data one may obtain and the notes on troubleshooting are invaluable to both seasoned investigators and novice students. (Elizabeth S. Raveche, Doody's Review Service, February, 2011)Serves primarily as a recipe book, detailing all the latest methods one would need to further investigate the roles of microRNAs in this area of biology. It will appeal to both students who are new to the field … and molecular biologists who can keep it close as a handy reference material for planning and carrying out their experiments. … appealing to anyone from beginners to experts. … an attractive book for those interested in identifying and characterising particular miRNAs in the immune system. (Daniel Peters, Immunology News, February, 2011), From the reviews: "This book is useful to scientists seeking to study the role of miRNAs in the immune system. ... helpful to immunologists, biochemists, and molecular biologists interested in miRNA effects. The detailed protocols are invaluable to students and post-docs in particular. ... The figures of the types of data one may obtain and the notes on troubleshooting are invaluable to both seasoned investigators and novice students." (Elizabeth S. Raveche, Doody's Review Service, February, 2011) "Serves primarily as a recipe book, detailing all the latest methods one would need to further investigate the roles of microRNAs in this area of biology. It will appeal to both students who are new to the field ... and molecular biologists who can keep it close as a handy reference material for planning and carrying out their experiments. ... appealing to anyone from beginners to experts. ... an attractive book for those interested in identifying and characterising particular miRNAs in the immune system." (Daniel Peters, Immunology News, February, 2011)
Series Volume Number
667
Number of Volumes
1 Vol.
Illustrated
Yes
Lc Classification Number
Qr180-189.5
Table of Content
Analysis of miRNA Expression: Classic Methods Revisited.- A Rapid, Quantitative Assay for Direct Detection of MicroRNAs and Other Small RNAs Using Splinted Ligation.- Normalization of MicroRNA Quantitative RT-PCR Data in Reduced Scale Experimental Designs.- MicroRNA Detection in Bone Marrow Cells by LNA-FISH.- Measuring MicroRNA Expression in Size-Limited FACS-Sorted and Microdissected Samples.- High-Throughput Analysis of miRNAs.- MicroRNA Cloning from Cells of the Immune System.- High-Throughput Profiling in the Hematopoietic System.- Construction of Small RNA cDNA Libraries for Deep Sequencing.- MicroRNA-Profiling in Formalin-Fixed Paraffin-Embedded Specimens.- Functional Analysis of miRNAs in the Immune System: Gain-of-Function.- Expression of miRNAs in Lymphocytes: A Review.- Mouse Models for miRNA Expression: The ROSA26 Locus.- Regulation of Monocytopoiesis by MicroRNAs.- MicroRNA Activity in B Lymphocytes.- Isolation and Characterization of MicroRNAs of Human Mature Erythrocytes.- Stable Overexpression of miRNAs in Bone Marrow-Derived Murine Mast Cells Using Lentiviral Expression Vectors.- Monitoring MicroRNA Activity and Validating MicroRNA Targets by Reporter-Based Approaches.- Functional Analysis of miRNAs in the Immune System: Loss-of-Function.- Lentivirus-Mediated Antagomir Expression.- miRNA Post-Transcriptional Modifications and Mechanisms of Action.- Solution Structure of miRNA:mRNA Complex.- MiRNA Editing.- Bioinformatic Analysis and Target Prediction.- Computational Prediction of MicroRNA Targets.- Large-Scale Integration of MicroRNA and Gene Expression Data for Identification of Enriched MicroRNA-mRNA Associations in Biological Systems.- miRNA and Viruses.- Identification and Validation of the Cellular Targets of Virus-Encoded MicroRNAs.
Copyright Date
2010

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